Study of G1 phase synchronization in endometrial carcinoma HEC-1-A cells
10.3760/cma.j.issn.1006-9801.2012.08.001
- VernacularTitle:子宫内膜癌HEC-1-A细胞G1期同步化研究
- Author:
Xiaomao LI
;
Huimin SHEN
;
Xiaoyun WANG
;
Xu ZHANG
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
Lovastatin;
HEC-1-A cell;
Cell cycle;
G1 phase synchronization
- From:
Cancer Research and Clinic
2012;24(8):505-507,511
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lovastatin on inducing G1 phase synchromzation in HEC-1-A cells and examine the cell cycle progression after desynchronization.Methods The doubling time of HEC-1-A cells was detected by cell counting Kit-8 assay.To determine the best lovastatin concentration of G1 synchronization,HEC-1-A cells were treated with lovastatin at concentration of 10,20,30 and 40 μmol/L respectively for 1 × doubling time,and the cell cycle was detected by flow cytometry (FCM).To determine the best period of lovastatin treatment to achieve G1 synchronization,HEC-1-A cells were treated with lovastatin at the best concentration for 0.5 × to 2 × doubling time,and the cell cycle was detected every 4 h using FCM.Furthermore,the cell cycle progress of HEC-1-A cells after desynchronization was also observed.Results The doubling time of HEC-1-A cells was 24 h.Treated with lovastatin at concentration of 40 μmol/L for 28 h achieved maximum G1 arrest (87.87±0.70) ％ in HEC-1-A cells.Minimum G1 phase (58.42±0.54) ％ and maximum S phase (33.58±0.62) ％ were observed after desynchronizing for 20 h.Conclusion Maximum G1 synchronization of HEC-1-A cells is induced by lovastatin at concentration of 40 μmol/L for 28 h.The HEC-1-A cells show minimum G1 phase and maximum S phase after desynchronizing for 20 h.
