A marine anthraquinone SZ-685C inhibits cell proliferation and induces apoptosis in GH3 cells
10.3760/cma.j.issn.1001-2036.2012.05.002
- VernacularTitle:新颖海洋化合物SZ-685C抑制GH3细胞增殖并诱导其凋亡
- Author:
Ni LEI
;
Weiwei XIAO
;
Zhigang MAO
;
Xiaobing JIANG
;
Yonghong ZHU
- Publication Type:Journal Article
- Keywords:
Pituitary adenoma;
SZ-685C;
Somatolactotroph cell;
Proliferation;
Apoptosis
- From:
Chinese Journal of Microsurgery
2012;35(5):356-359,442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of SZ-685C on rat pituitary adenoma GH3 cell line.Methods MTT method evaluated its effect of proliferation and inhibitory concentration 50 (IC5o) on GH3 cells,after treated by 0,2.5,5.0,7.5,10.0,12.5,15.0,17.5,20.0 and 30.0 μmol/L SZ-685C for 48 h,GH3 cells were changed to complete medium for 12 d,crystal violet staining was used to investigate colony formation; microscopy and Hoechst 33342 staining assay observed whether the changes of morphological as the result of apoptosis,then detected cell apoptosis by flow cytometry.Results SZ-685C had a dose-dependent inhibitory effect on GH3 cell proliferation and IC50 was (12.9 ± 0.47) μmol/L,MEF(considered as a control group) had little affect in cell proliferation on the concentration of IC50.Inhibitory effects persisted even on removal of SZ- 685C after 12 d,and SZ-685C blocked colony formation ability of pituitary tumor cells.Apoptotic morphological observation of microscope and Hoechst 33342 staining proved apoptosis during treatment of SZ-685C,flow cytometry detection showed that SZ-685C induces 36.4％ of apoptosis,while only 2.0％ in control group.Conclusion SZ-685C inhibits pituitary tumor cell proliferation and induces apoptosis.SZ-685C can be a new anti-patuitary tumor drug for a further study.
