Study on promotion of growth hormone for colorectal cancer cell proliferation
10.3760/cma.j.issn.1006-9801.2011.10.007
- VernacularTitle:生长激素促进结直肠癌细胞增殖的实验研究
- Author:
Xiaoyu WU
;
Liang GE
;
Zhiwei CHEN
;
Xuequan YAO
;
Che CHEN
;
Zhe XU
;
Weisu LI
;
Fukun LIU
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Growth hormone;
Growth hormone receptor
- From:
Cancer Research and Clinic
2011;23(10):671-673,677
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of GHR in colorectal cancer cell lines and determine whether recombinant human growth hormone can promote the proliferation of colorectal cancer cells in vitro.Methods GHR distribution was assessed by flow cytometry and immunofluorescence method in 9 colorectal cancer cell lines.The effect of recombinant human growth factor on colorectal cancer cell line proliferation was assessed by MTT method.Results Different GHR expression was determinated in 9 colorectal caner cell lines.GHR was highly expressed in HCT-8 while GHR expression could hardly be detected in LoVo.r-hGH could promote GHR(+) HCT-8 cell proliferation at 50 ng/ml and 100 ng/ml (P ＜0.05).But this effect was not dose dependent.When the neutralizing antibody was used to block GHR activity,this r-hGH dependent proliferation effect was eliminated.r-hGH could not promote GHR (-) LoVo cell proliferation (P ＞0.05).Conclusion The results demonstrates that r-hGH could promote GHR (+) tumor cell proliferation and this effect is mediated by GHR.The use of r-hGH on the colorectal cancer patients should be cautious.
