Continuously observing cutaneous vitality of DIEP flap in rat model
10.3760/cma.j.issn.1001-2036.2012.05.012
- VernacularTitle:大鼠腹壁下动脉穿支皮瓣动态血流观测模型的构建
- Author:
Wen CHEN
;
Yangqun LI
;
Yong TANG
;
Zhe YANG
;
Muxin ZHAO
- Publication Type:Journal Article
- Keywords:
Perforator flap;
DIEP flap;
Animal model;
SPY system
- From:
Chinese Journal of Microsurgery
2012;35(5):391-394,448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To carried out rat as deep inferior epigastic perforator (DIEP) flap necrosis model,to reveal the continuous blood fluid change in process of flap necrosis.Methods Seven SpragueDawley rats of male were used.The designed DIEP flap model was pedicled on the right-sided cranial perforator.Near-infrared fluorescent angiography was performed using SPY imaging system pre-and-aft operation and all angiography videos were compared and analyzed.Results Using SPY imaging system could observe the process of the blood fluid scattered from perforator through choke anastomoses to next vascular territories in living body.The study could clearly observe sequence change of blood fluid pr-and-aft operation,which could give the important information in revealing the reason of necrosis in DIEP flap.Cross-midline traffic vascular sparse and eventually could no longer wear through the contralateral abdominal wall vessel area and open in the region of lateral thoracic vascular occlusion of blood vessels to reach the flap to the far left side,and showed no blood flow in the dark zone.Conclusion The reasons of necrosis of contralateral distal zone of the flap model,are the choke anastomoses across the midline to the contralateral side appeared small,less intensity and the blood supply to contralatral distal zone should through twice choke anastomoses.