The α7 nicotine acetylcholine receptor regulates sensitivity of regular chemotherapeutic agent of cholangiocarcinoma

VernacularTitle:α7烟碱型胆碱能受体调节胆管癌细胞化疗敏感性的相关探讨
Author: Guangwei LIU ; Bingyuan ZHANG ; Yujie FENG ; Ruyong YAO ; Jian YU ; Kunye LUAN ; Kun LI ; Yun LU
Publication Type:Journal Article
Keywords: α7 nicotine acetylcholine receptor; Chemotherapy; Apoptosis; Cholangiocarcinoma; Resistance
From: International Journal of Surgery 2012;39(5):306-309,封3
CountryChina
Language:Chinese
Abstract: ObjectiveTo study the effect of α7 ( α7 AChR) agonist nicotine on regulating sensitivity of regular chemotherapeutic agent in cholangiocarcinoma cells,and explore the possible target.MethodsThe effect of nicotine and α-BTX pretreatment on the survival ability of cholangiocarcinoma cells was investigated when applied with 5-FU by using MTT and Flat cloning formation experiment.ResultsApplied with 5-FU,in various con centrations nicotine stimulating group( 10-3 g/L,10-4 g/L,10-5 g/L ),the survive rate of QBC939 was 128％,124％,118％,while that in α-BTX stimulating group and combined stimulation group was 92％,94％,93％,92％,respectively.The cloning formation ability of nicotine- stimulating group (6.2 ± 0.40) was significantly higher than α- BTX stimulating group (3.2 ± 0.20 ),combined stimulation group ( 3.2 ± 0.20 ) and control group ( 3.4 ±0.33).ConclusionNicotine can prevent chemotherapy-induced apoptosis,and improve cholangiocarcinoma cell survival via α7 nicotine acetylcholine receptor in vitro.