Screening serum markers of pancreatic cancer by 2D differential gel electrophoresis and MALDI-TOF-MS
10.3760/cma.j.issn.1673-4203.2012.05.009
- VernacularTitle:应用2D-DIGE及MALDI-TOF-MS筛选胰腺癌血清标志物
- Author:
Yunlian XIA
;
Jiong CHEN
;
Yin LU
;
Hangcheng ZHOU
;
Renbao YANG
;
Wen WU
;
Longjiang CHEN
;
Liwei HU
- Publication Type:Journal Article
- Keywords:
Pancreatic carcinoma;
Proteomics;
2D differential gel electrophoresis;
Matrix-assisted laser desorption/ionization time of flight mass spectrometry;
Markers
- From:
International Journal of Surgery
2012;39(5):313-317,封3
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveA comparative proteomic method was used to analyse serum proteins between pancreatic cancer patients and control group,and to find a new protential specific marker.MethodsComparative analysis on the pancreatic peripheral blood protein profiling from 40 pancreatic cancer patients,10 chronic pancreatitis patients,10 benign tumor patients and 40 cancer-free controls was carried out by 2D differential gel electrophoresis,and differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry.ResultsTwo differentially expressed proteins:transthyretin and apolipoprotein E were identified.Those proteins were highly expressed in pancreatic carcinoma group compared with normal control group,chronic pancreatitis group and benign tumor group.Conclusion2D differential gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry technology in screening specific serum biomarkers of pancreatic cancer has a well repeatability and stability.The identified protein transthyretin in this study may be as specific serum biomarkers of pancreatic carcinoma.
