Dynamics of IL-2 and IL-7 levels during Highly active antiretroviral therapy and their significance
10.3760/cma.j.issn.1008-1372.2012.03.006
- VernacularTitle:中国HIV感染者IL-2和IL-7参与高效抗反转录病毒治疗的抗病毒免疫反应及其在病毒控制中的作用
- Author:
Ma DIALLO
;
Xia CHEN
;
Yuhuang ZHENG
;
Yah HE
;
Bo HE
;
Huaying ZHOU
;
Zi CHEN
;
Yan LUO
;
Si ZENG
- Publication Type:Journal Article
- Keywords:
HIV irfections/IM;
Interleukin-2/IM;
Interleukin-7/IM;
HIV-1 /IM;
Antiretroviral therapy,highly active;
Viruses/DE/IM
- From:
Journal of Chinese Physician
2012;14(3):308-312,317
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this study was to investigate the course of certain common gamma cytokines ( IL-2 and IL-7 ) and their role on the control of the viral infection in a short term antiviral therapy.Methods A total of 35 adults with chronic HIV infection,responding to combined antiretroviral therapy (cART) guideline criteria were enrolled in this one year follow-up study.After signing an informed consent,20 ml blood were collected from each patient at base line,week 0,week 24 and week 48.1 ml serum collected from each patient was kept at -80 * C until use.Serum concentration of IL-2 and IL-7 was determined using ELISA kit from ebioscience Beijing.CD4 and CD8 cells were counted and quantified using flux cytometry,and serum HIV RNA was quantified using real time PCR.Results All patients had a mean baseline IL-2 level [ (9.67 ± 2.6 ) pg/ml ]lower than the controls [ ( 27.36 ± 5.05 ) pg/ml ].After treatment for 48 weeks,IL-2 increased[ ( 19.8 ± 3.3 ) pg/ml ].However,the mean baseline 1L-7 [ ( 81.74± 20.47 ) pg/ml ]in patients was higher than controls [ ( 2.06 ± 1.52 ) pg/ml ].After treatment for 48 weeks,IL-7 decreased [ (8.36 ± 2.16)pg/ml ].IL-2 showed a significant increase and positive correlation with CD4 cells after HAART initiation (0week:R =0.21,P =0.063,24week:R =0.24,P =0.033,48week:R =0.19,P =0.103; IL-7 showed a significant decrease after HAART initiation but it did not show correlation with CD4 cells.We noted there was a negative correlation between IL-2 and CD4 count in HAART baseline (R =0.28,P =0.012 ),but no correlation between IL-7 and CD4 count from 6 month after HAART.IL-2 showed negative correlation with HIV RNA ( R =- 0.17,P =0.032),but IL-7 showed a relationship with the HIV RNA Conclusions The increase of IL-2 coupled with the decrease of IL-7 revealed a partial restoration of immune response during HAART.However,the absence of relationship with HIV RNA suggested that these cytokines might not be directly involved in the reduction of viral load.
