Study on the mechanism of themolecular biology of hepatocyte growth factor and connective tissue factor in proliferative vitreoretinopathy
10.3760/cma.j.issn.1008-6706.2011.13.003
- VernacularTitle:靶向结缔组织生长因子shRNA慢病毒表达载体的构建及病毒包装
- Author:
Ying YU
;
Youxin CHEN
- Publication Type:Journal Article
- Keywords:
Intercellular signaling peptides and proteins;
RNA interference;
Lentivirus infections;
Pigment epithelium of eye;
Far-Western blotting
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(13):1733-1734,后插3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the role of CTGF in PVR, we successfully down - regulated the expression of CTGF by RNA interference( RNAi) technology in ARPE-19 cell line. Methods Design and synthesis three CTGF siRNA, screen effective targeted points in Western blot, combine Oligo DNA of effective targeted point, anneal to form double chain DNA, connect with linearing pGC-LV-GFP carrier, sequence DNA in PCR screening positive clones. Results The shRNA-expression vector of CTGF was constructed successfully. And RT-PCR and Western Blot results showed that CTGF expression was significantly inhibited by siRNA transfectants in ARPE-19 cells at mRNA and protein levels. Conclusion We successfully construct shRNA-expression vector of CTGF which paves a way for CTGF-targeted gene therapy of proliferative vitreoretinopathy.
