Development and application of HPLC method in simultaneous determination of mycophenolic acid and its 7-O-glucuronide metabolite in human plasma

VernacularTitle:高效液相色谱法同时测定霉酚酸及其葡糖苷酸的血浆浓度及临床应用
Author: Jiawen HUANG ; Yanfeng LIU ; Jiali LI ; Li WANG ; Changxi WANG ; Guoping ZHONG ; Min HUANG
Publication Type:Journal Article
Keywords: Mycophenolic acid; Glucuronates; Plasma concentration; Chromatography,high pressure liquid
From: Chinese Journal of Primary Medicine and Pharmacy 2011;18(13):1729-1731,后插3
CountryChina
Language:Chinese
Abstract: Objective To establish a high-performance liquid chromatography method for simultaneous determination of mycophenolic acid(MPA) and its 7-O-glucuronide metabolite (MPAG) in human plasma and to study pharmacokinetics of MPA in Chinese renal transplant recipients after administration of mycophenolate mofetil( MMF). Methods Plasma protein was precipitated with metlianol: 5% ZnSO4 ( 70∶30, V∶ V), using naproxen as internal MPA and MPAG after 1.5g/d administration of MMF in 6 early-stage renal transplant recipients were as follows :Tmax was(1.08±0.74)h and(2.58±1.24)h,Cmax was(21.33±8.61)mg/L and(106.98±31.91)mg/L,AUCO-t was (58.73 ±16.26)mg ? L-1 ? h-1 and(833.32±215.03)mg ? L-1 ? h-1,respectively.Conclusion This method was accurate, sensitive and specific and it was successfully applied in therapeutic drug monitoring (TDM) of mycophenolate mofetil in early-stage renal transplant recipients.