Transinfection of rabbit knee osteoarthritis models via chitosan microsphere as gene carriers with recombined human IL-IRa gene and TGF-β1 gene
10.3760/cma.j.issn.1001-2036.2011.03.012
- VernacularTitle:壳聚糖微球转染人IL-1Ra与TGF-β1基因治疗兔骨关节炎
- Author:
Xiaoyue WEN
;
Huading LU
;
Daozhang CAI
;
Yuxian CHEN
;
Kun WANG
;
Chun ZENG
- Publication Type:Journal Article
- Keywords:
Chitosan;
Interleukin-1 receptor antagonist;
Transforming growth factor β1;
Gene transinfection;
Osteoarthritis
- From:
Chinese Journal of Microsurgery
2011;34(3):207-210,后插4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the method and effect of transinfection of rabbit early knee osteoarthritis models via chitosan microsphere with gene of recombined human IL-1Ra gene and TGF-β1 gene. Methods Chitosan microspheres with plasmids of IL-1Ra gene and TGF-β1 gene, and rabbit early knee osteoarthritis models were prepared. Rabbits in different groups had intra-articular injections of chitosan microsphere containing IL-1Ra gene and / or TGF-β1 gene, and chitosan solution as control group before being executed regularly and randomly. The joint specimens were evaluated by HE staining, lycopene red O staining and immunohistochemical analysis and Mankin's score. ELISA was used for detection of IL-IRa and TGF-β1 concentration of articular cavity fluid in each group. Results The control group was consistent with the pathological changes of early OA. In co-transinfection group, judging from the appearance and staining of cartilage,the OA damage of the specimens was less serious than other groups'. Its Mankin's score was significantly lower than single-gene transinfection group (P ＜ 0.05), and the latters Mankin's score were significantly lower than control group (P ＜ 0.05). Conclusion Intra-articular injection of chitosan microspheres containing both IL-1Ra gene and TGF-β1 gene could inhibit the degeneration of cartilage and promote cartilage repair.
