High fat diet influence on vitamin D receptor expression and endothelial nitric oxide synthase in apolipoprotein E-deficient mice
10.3760/cma.j.issn.1008-1372.2011.04.001
- VernacularTitle:高脂饮食对载脂蛋白E缺乏鼠维生素D受体表达及一氧化氮合酶活性影响研究
- Author:
Wei XIANG
;
Xiaojie HE
;
Yanlin MA
;
Zhuwen YI
;
Yan CAO
;
Shuiping ZHAO
;
Jinfu YANG
;
Zhichao MA
;
Ming WU
;
Shengmiao FU
;
Jianlin MA
;
Jie WANG
;
Wei ZHENG
;
Hong KANG
- Publication Type:Journal Article
- Keywords:
Dietary fats;
Apolipoproteins E/ME;
Receptors,calcitriol/ME;
Nitric oxide synthase/BI
- From:
Journal of Chinese Physician
2011;13(4):433-436,440
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study investigated high fat diet influence on the changes of vitamin D receptor (VDR) expression and endothelial nitric oxide synthase (eNOS) in apolipoprotein E-deficient(apoE-/-) mice.MethodsApoE-/- mice and C57BLP6J mice were divide into two groups (normal control and high fat diet),high fat diet group were feed high fat feedstuff.Plasma 25-(OH)D levels were determined by competitive protein binding radioimmunity,VDR expression were determined by immunofluorescence and reverse transcription-polymerase chain reaction.The levels of NO and eNOS were determined by nitrate reductase.ResultsCompared with normal control group,high fat diet caused more severe dam-age of atherosclerosis in wild type mice and apoE-/- mice.In apoE-/- mice,the levels of plasma 25-(OH)D were significantly decreased [(26.44±1.28) ng/mL,(22.68±2.07)ng/mL,(17.46±2.22)ng/mL,(15.88±0.97)ng/mL,P＜0.01],the expression of VDR protein and mRNA were significantly increased[VDR :0.244±0.088,0.346±0.132,0.547±0.128,0.768±0.162;VDRmRNA:0.228±0.083,0.375±0.103,0.451±0.117,0.597±0.131,P＜0.01],and the levels of NO and eNOS were significantly increased[NO:(39.74±4.81)μmol/L,(48.1±5.24 )μmol/L,(67.34±6.14 )μmol/L,(86.74±8.05)μmol/L;eNOS:(8.6±0.77 )U/L,(12.28±1.42)U/L,(15.96±0.92)U/L,(18.68±1.15)U/L,P＜0.01].These changes were more significantly in high fat diet group(P＜0.01).ConclusionsThere were abnormalities of plasma 25-(OH)D level,VDR expression and the level of NO and eNOS in apoE-/- mice.These changes were more significantly in high fat diet group.
