Effect of Kupffer cells on CD4~+ CD25~+ T cells in schistosome granuloma
- VernacularTitle:Kupffer细胞对日本血吸虫病肉芽肿期CD4~+CD25~+T细胞的影响
- Author:
Jianghua YANG
;
Lei HE
;
Tao WANG
;
Jinsun YANG
;
Weishun HOU
;
Chuan SU
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
CD4~+ CD25~+T cell;
Kupffer cell
- From:
Chinese Journal of Schistosomiasis Control
2010;22(1):17-19,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of targeting Kupffer ceHs on CD4~+ CD25~+T cells in schistosome gramtloma.Methods A total of 30 six-to eight-week-old C57BL/6 female mice were divided into three groups equally,namely a control group,an infection group with S.japonicum cercariae(10 cercariae per mouse) and an infection group injected with GdCl_3 through the penile vein(15 mg/kg)twice perweek.After8 weeks of theinfection,the number of CD4~+ CD25~+T cells was detected by using flow cytometry and the number of Foxp3 was detected by using immunohistochemistry.For the detection of murine IL-4,IL-5,IL-10,TGF-β1 and IFN-γ,a DuoSet ELISA development kit was used.Results The number of CD4~+ CD25~+T ceils and the level of IL-10 decreased significantly in the infection group injected with GdCl_3 compared with the infection group.GdCl_3 treatment decreased Foxp3 production and the level of ALT,and reduced the inflammatory response in schistosome Granuloma.Conclusion Kupffer ceils Can regulate the response of CD4~+ CD25~+T cells in schistosome granuloma.
