n-3 PUFA can inhibit the apoptosis of intestinal epithelial cells of chronic rejection after small intestinal transplantation
- VernacularTitle:n-3多不饱和脂肪酸对大鼠小肠移植慢性排斥肠黏膜细胞凋亡的抑制作用
- Author:
Kun ZHAO
;
Haiyun ZHANG
;
Meng WANG
;
Ning LI
;
Yousheng LI
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
n-3 fatty acid;
Chronic rejection;
Apoptosis;
Granzyme B;
Perforin
- From:
Parenteral & Enteral Nutrition
2009;16(6):355-357,363
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The aim of our work was to investigate the effects of n-3 polyunsaturated fatty acids on apoptosis, granzyme B and perforin expression of intestinal epithelial cells of chronic rejection after small intestinal transplantation. Methods: Small bowel transplantation was performed and rats were divided into three groups: Group 1, Lewis-to-Lewis, group 2, F344-to-Lewis, dietary corn oil, Group 3, F344-to-Lewis, dietary fish oil. All recipients were killed after 16 weeks of posttransplantation. The apoptosis rate of mucosal cells were evaluated by flow cytometry. The expressions of granzyme B and perforin were analyzed by reverse transcriptase RT-PCR. Results: A high apoptotic rate was observed when the allografts demonstrated one or more histological features of chronic rejection. N-3 polyunsaturated fatty acids decreased the rate of the apoptosis and inhibitted the expressions of granzyme B and perforin. Conclusion: N-3 polyunsaturated fatty acids can suppress the chronic rejection in small intestinal transplantation.
