Effect of BRMS1 on the invasion and metastasis of ovarian cancer cells by RNA interference
10.3760/cma.j.issn.1006-9801.2011.07.011
- VernacularTitle:应用RNAi技术研究乳腺癌转移抑制基因1对卵巢癌细胞侵袭转移的影响
- Author:
Dongmei ZHOU
;
Xiujie SHENG
;
Siyuan LOU
;
Qicai LIU
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Neoplasm metastasis;
Breast cancer metastasis suppressor 1
- From:
Cancer Research and Clinic
2011;23(7):471-473,476
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of BRMS1 on the invasion and metastasis of ovarian cancer cells. Methods BRMS1 small interfering RNA (BRMS1-siRNA) was transfected into human ovarian cancer cell-line OVCAR3 by liposome transfection method. The cells were divided into 3 groups: experimental group with BRMSl-siRNA, negative control group with siRNA that did not impact any gene, blank control group without any transfection. Changes of invasion and migration in the cells were per-formed using transwell invasion and migration assay. Results BRMS1 gene was silenced in OVCAR3 cell line successfully detecting by quantitative real-time RT-PCR and immunoblotting.In transwell invasion assay,the numbers of cells in lower chamber passing through the membrane in transfected group were more than negative control group and blank control group (190±8.5, 144±7.8 and 146±6.8 respectively) (t=8.747, t=8.869, P=0.000), while in transwell migration assay, the numbers of cells in lower chamber passing through the membrane in transfected group were more than negative control group and blank control group were (231 ±8.9, 177±9.7 and 182±7.9 respectively) (t=9.314, t=9.224, P=0.000), both with significant differences among the 3 groups. Conclusion BRMS1 gene could suppress the invasion and metastasis of ovarian cancer cells.
