Expressions of endothelin-1 in primary cultured atrial myocytes during early rapid pacing

VernacularTitle:快速起搏大鼠心房肌细胞内皮素-1表达变化
Author: Yanxia LIU ; Po ZHANG ; Xianyang ZHU ; Xiumin HAN ; Duanzhen ZHANG ; Qiguang WANG
Publication Type:Journal Article
Keywords: Atrial fibrillation; Myocardium; Remodeling; Endothelin-1
From: Chinese Journal of cardiovascular Rehabilitation Medicine 2012;21(4):341-345
CountryChina
Language:English
Abstract: Objective: To study the expressions of endothelin-1 in primary cultured atrial myocyte model at early stages of atrial fibrillation by rapid pacing. Methods: The primary rat atrial myocytes were cultured, in which a rapid paced cell model was established. The polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were applied to detect the messenger ribonucleic acid (mRNA) expression and secretion of endothelin-1 on 3 h, 6 h, 12 h and 24 h after rapid pacing. Results: Compared with before pacing, there were continuous and significant increase in expression of ET-1mRNA [ (0.31±0.02) vs. (0.52±0.07), (0.62±0.09), (0.75±0.05) ] and secretion of endothelin-1 [ (3.4±0.8) ng/L vs. (6.0±1.4) ng/L, (8.3±1.5) ng/L, (11.2±2.1) ng/L] on 6 h, 12 h and 24 h after rapid pacing, P<0.01 all. Conclusion 　 Expressions of ET-1mRNA and secretion of endothelin-1 significantly increase depend on time in early rapid pacing atrial myocytes, indicating that endothelin-1 participates in atrial pathological remodeling during atrial fibrillation.
Full text:P020121009764850783143.doc