Detecting KRAS,EGFR mutations in nonsmall-cell lung cancer/colorectal cancer patients by high resolution melting curve analysis
10.3760/cma.j.issn.1008-6706.2011.04.003
- VernacularTitle:高分辨率熔解曲线分析法检测非小细胞肺癌/结直肠癌患者KRAS和EGFR基因突变
- Author:
Haiyan ZHANG
;
Ji WANG
;
Xiaodong CHEN
;
Yu CHEN
;
Lin ZHENG
- Publication Type:Journal Article
- Keywords:
Nonsmall-cell lung cancer;
Colorectal cancer;
Mutation;
KRAS gene;
EGFR gene;
High resolutionmelting curve analysis
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(4):439-441,后插3
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the feasibility of detecting the mutations of KRAS,EGFR in nonsmall-cell lung cancer and colorectal cancer patients with high resolution melting curve analysis. MethodsAt first,the mutations in KRAS exon 2 and EGFR exons 18 to 21 of 5 patients with non-small cell lung cancer and 5 patients with colorectal cancer were detected by high resolution melting curve analysis.Then the results were confirmed with direct sequencing. ResultsBy high resolution melting curve analysis,1 of 10 patients was found to carry EGFR 19 mutation who was harboring 2236-2250del mutation.By direct sequencing,consistent results of the patients with mutations orwithout mutations were got. Conclusion s The new high resolution melting curve analysis was more efficient,more convenient than direct sequencing.Moreover,it was a low-cost test,which was suitable for clinic test.
