Elevated MCP-1 stimulated with Mtb-specific peptides in patients with pulmonary tuberculosis

VernacularTitle:肺结核患者外周血单个核细胞MCP-1表达上升
Author: Xinjing WANG ; Zhihong CAO ; Bingfen YANG ; Yanhua LIU ; Jing JIANG ; Xiaoxing CHENG
Publication Type:Journal Article
Keywords: Tuberculosis,pulmonary/BL/ME; Chemokine CCL2/ME
From: Journal of Chinese Physician 2011;13(3):322-323,330
CountryChina
Language:Chinese
Abstract: Objective To study the feature of MCP-1 in plasma of active pulmonary tuberculosis patients and the correlation of MCP-1 obtained from PBMCs stimulated with specific Mtb peptides with IFNγ.Methods 20 patients with active pulmonary tuberculosis and 16 healthy controls with positive PPD were enrolled.The concentration of MCP-1 and IFN-γwas analyzed with Bender Flowcytomix on flow cytometry.Results No statistical difference of MCP-1 concentration in plasma was found between TB patients and controls [(263.8 ± 25.31)pg/ml and(212.1 ± 18.04)pg/ml,P ＞ 0.05].But TB patients with continuous respiratory symptoms showed higher MCP-1 in plasma.The concentration of MCP-1 and IFN-γwas significantly elevated in PBMCs culture supernatants.It was significantly higher in TB patients than in controls [(21460 ±3376)pg/ml vs(10910 ±2141)pg/ml,P ＜0.01].There was no correlation between the concentration of MCP-1 and IFN-γ.Conclusions The concentration of MCP-1 in plasma may be related to the progress of the pulmonary tuberculosis.MCP-1 stimulated by Mtb-specific peptides may be one of the biomarkers for TB diagnosis.