The study of immune response gene and the mechanism of the membranous glomerulonephritis in the mouse
10.3760/cma.j.issn.1008-6706.2011.05.012
- VernacularTitle:免疫应答基因与小鼠膜性肾小球肾炎发病的关系
- Author:
Huxiang ZHANG
;
Haiyan ZHANG
;
Li WAN
;
Yuerong MA
- Publication Type:Journal Article
- Keywords:
Genes,MHC Class H;
Reverse transcriptase polymerase chain reaction;
Glomerulonephritis,membranous
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(5):602-604,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immune response gene and the mechanism of membranous glomerulonephritis in the mouse. Methods Reproduced and identitied the animal model of MGN in mice ,extracted the total RNA of pathology group and the control group,amplified and verified I-Aβ1 gene through RT-PCR. Then sequenced and analyzed the I-AβI gene from the PCR production. Results The mutation rate of the I-Aβ1 gene was 2. 578‰ in the pathology group, and 0. 286‰ in the control group. It was obviously higher in the pathology group than in the control group(P ＜0.01). Conclusion I-Aβ1 gene rose in mice,may be related to membranous glomerulonephritis.
