The expression of connective tissue growth factor in asthmatic mice and the effect of azithromycin
10.3760/cma.j.issn.1008-6706.2011.12.005
- VernacularTitle:结缔组织生长因子在哮喘小鼠肺组织中的表达及阿奇霉素的干预作用
- Author:
Meimei ZHENG
;
Fangjian WANG
;
Yong JIANG
;
Wujin WEN
;
Chengcheng DUAN
;
Kechong DENG
- Publication Type:Journal Article
- Keywords:
Bronchial asthma;
Connective tissue growth factor;
Asthma airway remodeling;
Azithromycin
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(12):1593-1595,后插一
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of connective tissue growth factor( CTGF) and the effects of azithromycin in airway remodeling in asthmatic mice.Methods BALB/c female mice were randomly divided into 3 groups;control group(A),asthma group(B) ,and azithromycin treated group(C),with 10 mice in each group.Mice were sensitized and challenged continually with ovalbumin( OVA).The distribution and types of collagens were detected by cirius red staining.Lung hydroxyproline content was determined by acid hydrolysis.CTCF expression in mice pulmonary tissues was detected by SABC immunohistochemistry.Results After continual challenging with OVA,collagen hyperplasia was found in the airway wall and CTCF positive expression in airway epithelium.CTCF expression was closely associated with lung hydroxyproline content(r = 0.65,P <0.01).Conclusion Asthma continually challenged with OVA could result sub-epithelial fibrosis and remodeling in airway,which could be associated with the upregulation of CTCF over-expression.Azithromycin inhibited airway remodeling in asthmatic mice,possibly by inhibiting the expression of CTGF.
