Effects of Ketamine on Ca2 + Transsarcolemmal Influx in Isolated Rat Ventricular Mycocytes
- VernacularTitle:氯胺酮对大鼠心肌细胞钙离子移动的影响
- Author:
Ying ZHANG
;
Shitong LI
;
Xinliang ZHUANG
;
Jichang LI
- Publication Type:Journal Article
- From:
Fudan University Journal of Medical Sciences
2001;28(1):61-62,65
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the effects of different concentration of ketamine on Ca2 transsarcolemmalinflux induced by KCl in isolated rat ventricular myocytes. Methods Freshly isolated rat ventricular nyoeyteswere loaded with Fluo-3AM, a Ca2 + indicator. The effects of different concentration ketamine( 1 × 10- s, 1 × 10- 4,1 × 10-3 mmol/L) on the change of intracellular Ca2+ concentration induced by KCl were investigated. ResultsLow concentration ketamine(1 × 10-5 mrnol/L) did not change Ca2+ transsarcolemmal influx. Although mediumeoncentration ketamine( 1 × 10-4 rmol/L) made the influx slower, the eventual peak concentration of intracellularCa2+ had no difference from that of the control group. The high concentration ketamine (1 × 10-3 mmol/L) inhibited Ca2-1 influx,intracellular Ca2+ fluorescent intensity decreased about 13.2% (P＜0.05). ConclusionsKetamine inhibits Ca2 + trranssarcolemmal influx in isolated rat ventricular myocytes dosedependently, which may inpart explain its negative inotropic effect.
