Expression of bcl-2 in Mareks disease tumor
- Author:
Shuxia ZHANG
;
Wanfang CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(8):729-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Expression of bcl-2 in Marek's disease (MD) tumor was analyzed and relationship between bcl-2 gene and lymphomagensis clarified. METHODS: Model of MD tumor was reproduced on SPF White Leghorn chickens, collected tumor and normal tissues, conserved in liquid nitrogen. Total RNA in the tissues was obtained by AGPC method, detected their concentration and analyzed by 1% denatured agrose gel containing formaldehyde. A plasmid (pTTCB1) that contains chicken bcl-2 1.2 kb fragment was amplified, extracted and digested with restriction endonuclease (RE), the 1.2 kb fragment was recoveried and puarified with purigene kit, labeled probes with [α-32P]. Expression of bcl-2 was analyzed with Dot blot and Northern blot hybridization. RESULTS: (1) There are bcl-2 transcripts in MD tumor and relevent normal tissues; (2) Expression of bcl-2 in MD tumor is much higher than that in normal tissues. CONCLUSION: Our findings indicated that expressive products of chicken bcl-2 promotes lymphomagenesis of MD by blocking apoptotic cell death.
