Production of enzymatic hydrolysate of Acetes chinensis with angiotensin Ⅰ -converting enzyme inhibitory and its antihypertensive effects on spontaneously hypertensive rats
- VernacularTitle:中国毛虾ACE抑制产物的酶法制备及其降低原发性高血压大鼠血压的效果
- Author:
Wenhong GAO
;
Xiaoming QIN
;
Chaohua ZHANG
;
Pengzhi HONG
;
Hongwu JI
;
Changyun WANG
- Publication Type:Journal Article
- Keywords:
Acetes chinensis;
enzymatic hydrolysis;
angiotensin I-converting enzyme inhibitory activity;
antihypertensive effects
- From:
Chinese Journal of Biochemical Pharmaceutics
2005;26(6):339-343,346
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To produce enzymatic hydolysates with angiotensin Ⅰ-converting enzyme (ACE) inhibitory activity and antihypertensive activity from Acetes chinensis. Methods ACE inhibitory activity of the hydrolysates of Acetes chinensis by five commercial proteases were determined in vitro to select a protease as the enzyme used in the preparation of enzymatic hydrolysate of Acetes chinensis, and orthogonal trials were employed to optimize its hydrolysis parameters, spontaneously hypertensive rats (SHR) were used to assess in vivo the hypotensive effects of hydrolysate under optimized condition. Results The hydrolysate with IC50 being0.65 mg/ml was obtained under the optimized condition of pH 2.4, 41℃, 3 h hydrolysis time, 3% enzyme/substrate ratio (E/S) and 8 % substrate concentration, and a recorded SBP reduction of 3 886.3 Pa (29mmHg) at 4 h after administration (1.0 g/kg BW) was observed. Conclusion Peptic hydrolysate of Acetes chinensis shows significant ACE inhibitory activity and antihypertensive activity.
