Clinical application of CCND1 gene expression detection with fluorescent quantitative polymerase chain reaction in the diagnosis of breast cancer
- VernacularTitle:荧光定量聚合酶链反应检测CCND1基因扩增在乳腺癌诊断中的应用
- Author:
Jun DENG
;
Wenfang HUANG
;
Shaoqin RAO
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Cyclin D1;
Breast neoplasms
- From:
International Journal of Laboratory Medicine
2008;29(9):772-773,776
- CountryChina
- Language:Chinese
-
Abstract:
Objective To use fluorescent quantitative polymerase chain reaction (FQ-PCR) todetect the level of cyclin D1 gene (CCND1), and to investigate its clinical application in the diagnosisand monitoring of breast cancer. Methods FQ-PCR was applied to detecting the expression level ofCCND1 gene in peripheral blood from 15 healthy females, 30 patients with benign breast disease and81 patients with breast cancer with β2-microglobin as the internal control. Results There were no sig-nificant difference in the level of CCND1 expression between healthy control group and benign breastdisease group (P>0. 05). The level of CCND1 expression was significantly higher in breast cancergroup than that in healthy control group and benign breast disease group (P<0.05). The difference inβ2-microglobin was statistically insignificant among three groups (P> 0. 05). The positive rate was45.7% in breast cancer group, and 0 in benign breast disease group. Conclusion FQ-PCR is a rapid,sensitive and specific method for quantitative determination of CCND1 gene, which may he used as anavailable tool for the diagnosis, monitoring of therapeutic effect and metastasis as well as prognosis e-valuation of breast cancer.
