Effects of Genistein-magnetic-nanoparticles on the Expression of FAK in Human Hepatocellular Carcinoma Cell line HepG2
- VernacularTitle:Genistein-磁性纳米微粒对人肝癌细胞中FAK表达的影响
- Author:
Gongping WANG
;
Dongpeng LI
;
Xiubao LIU
;
Yuyuan ZHAO
;
Xiaoshan FENG
- Publication Type:Journal Article
- Keywords:
Genistein;
Hepatocellular carcinoma;
Focal adhesion kinase;
Reverse transcription polymerase chain reaction
- From:
Journal of Medical Research
2009;38(8):25-27,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Genistein - magnetic - nanoparticles at different concentrations on the growth and ex-pression of focal adhesion kinase (FAK) in human hepatocellular carcinoma cell line HcpG2. Methods Activities of HepG2 cells treated by Genistein - magnetic - nanoparticles were examined by MTT assay. The expression of FAK mRNA and protein was respectively detected by immunohistochemistry staining and reversed transcriptase polymerase chain reaction (RT - PCR) . Results Genistein - magnetic -nanoparticles at a concentration of 10 -80mg/L inhibited the proliferation of HepG2 cells, with obvious concentration - dapendent and time - dependent effect relationships (P < 0.05). After treatment with various concentrations of Genistein - magnetic - nanoparticles for 48h, the relative level of FAKmRNA of Genistein - magnetic - nanoparticles and control groups was 1.242 ± 0.031,1.213 ± 0.443,0.834 ± 0.044,0.652 ± 0.039,0.446 ± 0.041, and 1.443 ± 0.053 (F = 21.97 ,P < 0.05), respectively. The expression of FAK protein in the cells was decreased, which showed an obvious a concentration - effect relationship. Conclusion Genistein - magnetic - nanoparticles can reduce the mRNA and protein expressions of FAK and inhibit the proliferation of HepG2 cells.
