The effects of CD28 superagonist antibody in rat obliterative airway disease
- VernacularTitle:CD28超抗体对大鼠闭塞性气管病的治疗作用
- Author:
Yongsheng NIU
;
Huimin FAN
;
Hao CAO
;
Gang LIU
;
Qian SHI
;
Zhongmin LIU
- Publication Type:Journal Article
- Keywords:
Tracheal transplantation;
Regulatory T cells;
Obliterative airway disease;
CD28
- From:
Chinese Journal of Immunology
2009;25(11):1038-1040,1044
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of superagonistic CD28~- specific monoclonal antibody JJ316 (supCD28 MAb) on preferentially expanded rat CD4~+CD25~+Treg (Treg) cells in vivo and its applicability in obliterative airway disease (OAD).Methods:The heterotopic tracheal transplantation model in rats was used.One group received mIgG-treatment(0.5 mg/rat) as control.The experimental group was treated with supCD28 Mab(0.5 mg/rat) via intraperitoneal injection on the day of transplantation.The changes of Treg cell population in cervical lymph nodes were monitored by flow cytometry after 7 days.Tracheas were harvested after 21 days for further histologic evaluation.Results:SupCD28 MAb administration was revealed with a significant increase in the CD4~+CD25~+ T and CD4~+Foxp3~+ T cells population in cervical lymph nodes compared to treatment with mIgG group on day 7 after transplantation,[8.5%±3.4% and 11.5%±2.7% (P<0.05 vs mIgG group)in the supCD28 Mab group,1.8%±1.9% and 3.2%±2.1% in the mIgG group,respectively].Furthermore,the allografts from animals treated with supCD28 MAb were significantly less airway obliteration and destruction of the epithelium compared with that of control group animals on day 21 after transplantation.Conclusion:SupCD28 MAb targets expansion of Treg cells and attenuates airway lumen obliteration in rat obliterative airway disease.
