Effects of photochemotherapy on angiogenic ability of endothelial cells and expression of integrin
- VernacularTitle:光化学疗法对内皮细胞管腔形成能力及整合素表达的影响
- Author:
Hui DENG
;
Dingfen YUAN
;
Chunlin YAN
;
Kanghuang LIAO
- Publication Type:Journal Article
- Keywords:
psoriasis;
angiogenesis;
photochemotherapy;
integrin
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2009;29(10):1174-1177,1190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of photochemotherapy on the angiogenic ability of endothelial cells and expression of integrin. Methods In vitro angiogenesis assay ( UVA exposure dose: 0, 2.0, 5.0 J/cm~2) was used to detect the effects of photochemotherapy on the angiogenic ability of endothelial cells. Reverse polymerase chain reaction and flow cytometry were employed to determine the effects of photochemotherapy on the expression of integrin mRNA and protein, respectively. bFGF stimulation group and PMA stimulation group were divided according to the inductors, and subgroups were divided according to the UVA exposure doses of 0, 2. 0 and 5. 0 J/cm~2 in each group. Results Combination of UVA (2.0 and 5.0 J/cm~2) and 8-MOP (100 ng/mL) resulted in a decrease in the angiogenic ability of endothelial cells in vitro and the expression of integrin mRNA and protein. Conclusion Photochemotherapy may inhibit the angiogenic ability of endothelial cells through downregulating the expression of integrin.
