Establishment of radioresistant human nasopharyngeal carcinoma cells and the differential gene expression by cDNA microarray analysis
- VernacularTitle:放射抗拒性鼻咽癌细胞系的建立及差异表达基因
- Author:
Yali WANG
;
Xijing WANG
;
Zhongwei WANG
;
Yingying JIN
;
Yi LI
- Publication Type:Journal Article
- Keywords:
nasopharyngeal carcinoma;
cDNA microarray;
radioresistance
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2009;30(6):741-745,758
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the differential gene expression in human nasopharyngeal carcinoma (NPC) cell line with different radiosensitivity by cDNA microarray analysis. Methods A radioresistant cell line, CNE-2R, was established from a human nasopharyngeal carcinoma cells line CNE-2 by repeated X-ray irradiation. The differential gene expression of CNE-2 and CNE-2R was screened with cDNA microarray by BioStarH-141s profile gene chip. Results There were expressed 308 genes to be screened out between CNE-2R cell line with different radiosensitivity and its parental CNE-2 cell line, while 176 up-regulated genes in CNE-2R cells and 132 down-regulated genes were found. In them, there were 40 up-regulated ones and 36 down-regulated ones whose ratios were higher than 6.0 or lower than 0.1. The different genes included DNA damage- and repair-related genes; cell cycle-related and cytoskeleton-related proteins; and apoptosis-related genes. Conclusion The radioresistant CNE-2R cells were isolated from the CNE-2 cell line by repeated X-ray irradiation. The stable radioresistance is the result of co-effect by polygene and multiple factors, which provide several gene targets to sensitize the radioresistant cells for improving the radiocurability of NPC.
