Effect of Emodin on intracellular calcium concentration ([Ca~(2+)]i) and apoptosis of hepatic cells after simulated cold ischemia-reperfusion
- VernacularTitle:大黄素对模拟冷缺血再灌注后肝细胞内钙离子浓度及细胞凋亡的影响
- Author:
Xiang QI
;
Yi Lü
;
Naiying SHEN
;
Chang LIU
;
Xuemin LIU
;
Bo WANG
- Publication Type:Journal Article
- Keywords:
Emodin;
hepatic cell;
ischemia-reperfusion injury;
intracellular free calcium concentration;
apoptosis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2009;30(6):669-671,676
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Emodin on intracellular calcium concentration ([Ca~(2+)]i) and apoptosis of hepatic cells after simulated cold ischemia-reperfusion. Methods Glucose-oxygen deprivation, low temperature, subsequent reoxygenation and rewarming were used to induce ischemia-reperfusion injury model in cultured hepatic cells which were divided into 4 groups: control group and Emodin-treated group(100, 10 and apoptosis rate were determined by flow cytometry (FCM) respectively; the content of lactate dehydrogenase (LDH) in supernatant was tested. Results Intracellular calcium fluorescence intensity in Emodin-treated groups of high, medium and low density was 24.12±0.51, 26.35±1.34 and 39.12±1.94, respectively, which were significantly lower than 105.29±1.01 in control group(P<0.01). Apoptosis rate in Emodin-treated groups of high, (179.67±18.57)u/L in Emodin-treated groups of medium and high density respectively, which were significantly lower than (351.33±34.16)u/L in control group(P<0.01). Conclusion Emodin could reduce [Ca~(2+)]i and inhibit apoptosis of hepatic cells after simulated cold ischemia-reperfusion, thus protecting hepatic cells effectively.
