Determination of predominance amino acids specific for McAb2 to Fba protein of GAS
- VernacularTitle:A族链球菌(GAS)Fba蛋白单克隆抗体对应表位核心氨基酸的确定
- Author:
Yiyang GUO
;
Cuiqing MA
;
Peng WEI
;
Xiurong WANG
;
Huidong FENG
;
Wanyi YAN
;
Lin WEI
- Publication Type:Journal Article
- Keywords:
Group A Streptococcus;
Fba protein;
Monoclonal antibody;
Epitope;
Phage peptide library
- From:
Chinese Journal of Immunology
2009;25(12):1059-1062,1066
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify monoclonal antibody McAb2 recognizing epitope of Fba of GAS.Methods:The overlapped peptides were synthesized and their abilities to bind McAb2 were detected by dot-ELISA.The predominance amino acids specific for McAb2 were screened using phage 7 peptide library.Results:The result by dot-ELISA analysis demonstrated that the synthetized peptide,amino-acid residues 100-112~(th),could bind McAb2 with high affinity.The predominance amino acids specific for McAb2 were ITPDL,which was located in 100-110~(th)aa of Fba by panning with phage 7 peptide library.Conclusion:The domain and the predominance amino acids of Fba recognized by McAb2 is determined.The results would contribute to the research of the role of Fba on the pathogenic mechanism of GAS,the identification of function of McAb2,and the development of epitope-peptide vaccine.
