Immunoenhancement effect of ICA on the mouse model of Cy-induced hypoimmunity
- VernacularTitle:淫羊藿苷(ICA)对化疗后免疫抑制小鼠的免疫促进作用
- Author:
Lianmei ZHAO
;
Xin JI
;
Xiaoming PAN
;
Qian ZHANG
;
Lina HAN
;
Baoen SHAN
- Publication Type:Journal Article
- Keywords:
ICA;
Cy;
Immunoenhancement;
Mice
- From:
Chinese Journal of Immunology
2009;25(12):1092-1095,1099
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Effect of ICA (Icariin) was observed on immunosuppression mice caused by Cyclophosphamide(Cy).Methods:A total of 60 mice,expect control group mice,were injected Cy intraperitoneally [300 mg/(kg·d)],then randomly divided into modelgroup(saline),positive (shenqi,1 ml/d)groups,ICA groups[150,80,40 mg/(kg·d)].Saline,shenqi and ICA were perfused respectively for 10 successive days;All mice were sacrificed after 12 h of last administration for performing following experiments.Measuring SI and TI and calculating amounts of lymphocyte from spleen and peripheral blood;Proliferative reaction of splenic lymphocyte stimulated by ConA and LPS were detected by MTT assays.Killing activity of NK and CTL cells for colon26 and level of TNF-α production were measured by LDH method.Ratios of CD3~+ T and NKT in splenic lymphocyte (SPL) were studied by FACS.Results:ICA can recovery TI and SI of mice to normal level and boost the population of splenic lymphocyte (SPL) (P<0.01).After treatment with ICA,activities of NK cell and CTL cell were significantly improved,and the level of TNF-α was significantly elevated.The ratios of NKT and CD3+T in the ICA-treated group were increased obviously (P<0.01).Conclusion:ICA can enhance immunological function and alleviate the side effect of chemotherapy effectively.
