Prokaryotic expression,purification and polyclonal antibody preparation of putative cytadhesin protein (PvpA) of Mycoplasma gallisepticum
- VernacularTitle:鸡毒支原体黏附蛋白PvpA的原核表达与纯化
- Author:
Hongxia JIANG
;
Jirong CHEN
;
Zhenling ZENG
;
Hualing YAN
;
Xuning LI
- Publication Type:Journal Article
- Keywords:
Mycoplasma gallisepticum;
putative cytadhesin protein;
prokaryotic expression;
Western blotting
- From:
Chinese Journal of Veterinary Science
2009;29(7):864-867,881
- CountryChina
- Language:Chinese
-
Abstract:
The PCR product of PvpA gene was cloned into prokaryotic expression vector pET41a(+) and the recombinant expression vector was then transformed into E.coli DH5a after identified by restriction enzyme digestion and PCR.The positive recombinant plasmid was transformed into E.coli BL21 (D3) and induced to express PvpA protein.The obtained protein was analyzed by SDS-PAGE and Western blotting,purified by Ni-NTA affinity chromatography.The results showed that the purified PvpA fusion protein was obtained successfully.The expressed protein reacted to the high anti-PvpA immune serum from rabbit specially by western blotting.This study would be helpful to established a new diagnostic method for the detection of M.gallisepticum.
