Impact of Smad3 Gene Knockout Bone Marrow Transplantation on Mice
- VernacularTitle:Smad3基因剔除的骨髓细胞移植对小鼠的影响
- Author:
Jing CHEN
;
Hong SHEN
;
Yong ZHAO
- Publication Type:Journal Article
- Keywords:
Smad3~(-/-);
bone marrow;
Activated T cells;
Transplantation;
bone marrow;
Mouse
- From:
Acta Laboratorium Animalis Scientia Sinica
2010;18(1):9-12,彩3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of T cells and disease development in the mice with Smad3 deficiency in the bone marrow cells.Methods Bone marrow cells obtained from Smad3 null(Smad3~(-/-))mice and wild type(Smad3~(+/+))mice were injected to Co~(60)-irradiated GFP mice,respectively.The general states of the bone marrow recipients were observed.The mice were sacrificed at the sixth week and the histopathological changes in the intestines were examined.The changes of T cells from lymph nodes were detected by flow cytometry.Results Smad3~(-/-) bone marrow recipient mice appeared a wasting syndrome and intestinal inflammation.The amount of CD4~+ CD62L~(lo) T cells in lymph nodes was significantly increased.Conclusion These results indicate that the mice with Smad3 deficiency in the bone marrow cells present an inflammatory disorder and their T cells are activated.
