Expression and effect of TGF-β1 and HSP47 on gluteal muscle contracture
- VernacularTitle:臀肌挛缩症发病过程中TGF-β1和HSP47的表达及其作用
- Author:
Chenguang ZHAO
;
Xijing HE
;
Bin LU
;
Haopeng LI
- Publication Type:Journal Article
- Keywords:
gluteal muscle contracture;
fibrosis;
TGF-β1;
HSP47
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2010;31(2):221-223,238
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression and effect of transforming growth factors-β1 (TGF-β1) and heat shock protein (HSP47) on gluteal muscle contracture (GMC). Methods We collected contraction band and adjacent muscle from GMC patients and explored the expression of TGF-β1 and HSP47 using immunohistohemistry, reverse transcription and polymerase chain reaction (RT-PCR), and Western blot analysis. Results TGF-β1 and HSP47 were intensely expressed in fibroblast cells and vascular endothelial cells. The expression of them increased 8.1-fold and 3.6-fold at the mRNA level, respectively (P<0.05). The same changes were found at the protein level, which increased 11.2-fold and 7.6-fold, respectively (P<0.05). Conclusion Up-regulation of TGF-β1 and HSP47 may initiate fibrotic cascade in the gluteal muscles of GMC patients.
