Study of Relationship between Hsp70 and Endothelial Activation in Placental Vascular Diseases
- VernacularTitle:热休克蛋白70与胎盘血管病中内皮激活的相关研究
- Author:
Yanxia LIU
;
Jian XU
;
Xin WANG
- Publication Type:Journal Article
- Keywords:
Gene arrays;
Heat shock protein70;
Endothelial activation;
Placental vascular disease
- From:
Journal of Practical Obstetrics and Gynecology
2010;26(3):222-224,前插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The pathogenesis of placental vascular disease(PVD) was further investigated. Methode:PVD group (study group) and normal pregnancy (control group) were recruited. Two-channel mi-croarray technique was applied to screen the most differently expressive genes of human genome, and veri-fied the different expressions in mRNA and protein levels by quantitative PCR and Westem-blot, also local-ized the expression of different genes in placental tissue by immunohistochemistry. Reeults:HspA6 and Hs-pA1A had extremely higher expression in placentas with PVD than normal pregnancy. These genes both en-coded Hsp70. Hsp70 was mostly expressed in endothelial and smooth muscle cells of placental microves-ssls. The expression of Hsp70 mRNA and protein were up-regulated in placental tissue and microvascular endothelial cells from PVD group compared with normal pregnancy ( P <0.05) ;The expression of Hsp70 mR-NA and protein had negative correlation with infant birth weight ( P < 0.05). Conclueione:Hsp70 was associ-ated with endothelial activation in the pathogenesis of PVD by initiating an innate immune response and in-flammatory reaction.
