The altered expression of apoptosis-related genes and its clinical significance by siRNA-Ku70 silencing in drug-resistant lung cancer cell line
10.3969/j.issn.1000-484X.2010.04.006
- VernacularTitle:沉默Ku70后人耐药肺癌细胞凋亡基因组表达变化及临床意义
- Author:
Yarong LI
;
Jie ZHANG
;
Ke WANG
;
Bing LIU
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
Drug resistance;
Apoptotic gene;
Ku70;
siRNA-Ku70
- From:
Chinese Journal of Immunology
2010;26(4):312-314,319
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Ku70 silencing on the expression of apoptosis-related genes in human NSLC cell line,A549 and drug-resistant A549DDP.Methods:Expression of Ku70mRNA in human A549 cells was examined by RT-PCR.Expression of apoptosis-related genes was examined by PCR-Array assay.Results: The Ku70mRNA was higher in A549DDP cells than in A549 cells.The expression of BAX,TP53 and TP73 was significantly increased whereas the expression of TNFRSF1A and BFAR decreased in siRNA-Ku70 A549DDP cells when compared to those of A549DDP cells.Conclsion:The amount of Ku70 mRNA is higher in A549DDP cells,suggesting that Ku70 is important for drug-resistance; Silencing Ku70 might reverse the drug-resistance in A549DDP cells through regulation of expression of apoptofic genes.
