Effects of Percutaneously Puncturing Vertebrate Adjacent to Cartilage Endplate and Injecting Pingyangmycin on Lumbar Intervertebral Disc and Cartilage Endplate in Rabbits
- VernacularTitle:经皮穿刺兔腰椎椎体内注射平阳霉素对腰椎软骨终板及椎间盘的影响
- Author:
Changlong HOU
;
Jianyong YANG
;
Wenquan ZHUANG
;
Huishuang FAN
;
Guosheng TAN
;
Wenbo GUO
;
Wei CHEN
;
Zhongwei ZHANG
;
Lijuan MAO
- Publication Type:Journal Article
- Keywords:
cartilage endplate;
intervertebral disc;
degeneration;
magnetic resonance imaging;
histology
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2010;31(2):221-224,237
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]This study was designed to evaluate the effects of percutaneously puncturing vertebrate adjacent to cartilage endplate and injecting pingyangmycin on lumbar intervertebral disc and cartilage endplate in New Zealand Rabbits.[Methods]Thirty-six New Zealand white rabbits were enrolled in this study.The fifth lumbar vertebmte(L_5)was injected with pingyangrnycin as experimental group,and the fourth lumbar vertebmte(L_4)injected normal sodium as control group.Six rabbits were selected randomly,then MRI and histological observation was performed in the first,second,third,fourth,Fifth week and third month after operation respectively.Moreover,the correlation analysis was performed between MRI and histological measurements for areas of the lesion in L_5.[Results]There was no obvious changes on MRI and histological examination in control group.For experimental group,there were also no obvious changes in the first two weeks after bperation.However,in the third week,it demonstrated slightly hyperintense signal on T_2WI and fat-suppression T_2WI(FS T_2WI),while FS T_1WI was hypointense signal.The signal changed more obviously in the fourth week.Histologically,the structure of vertibrates arranged disordedy,chondrocyte of endplate decreased and architecture became disorder.Anulus fibrosus and nucleus pulposus did not change.The cartilage endplate and intervertebral disc degenerated in the fifth week.Both of them degenerated more obviously in third month.There was a strong correlation between MRI and histological measurements for areas of the lesion in the fourth week(r=0.965,P＜ 0.001).[Conclusion]Degeneration of lumbar intervertebral disc and cartilage endplate in New Zealand Rabbits can be induced by percutaneously puncturing vertebrate adjacent to cartilage endplate and injecting pingyangmycin.
