Characterization of the ultrastructure and cytoskeleton of bone marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus
- VernacularTitle:系统性红斑狼疮患者骨髓间质干细胞超微结构及细胞骨架的特征
- Author:
Zhifeng GU
;
Shengnan ZHAO
;
Huayong ZHANG
;
Ting XU
;
Ouyang JIN
;
Kangxing ZHOU
;
Haiou LIU
;
Tianyi ZHANG
;
Lingyun SUN
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Mesenchymal stem cells;
Ultrastructure;
Actin;
Vinculin
- From:
Chinese Journal of Rheumatology
2008;12(6):397-400,插3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore ultrastructure and cytoskeleton characteristics of bone marrow-deftved mesenchymal stem cells (MSCs) in patients with systemic lupus erythematosus (SLE).Methods MSCs were isolated from bone marrow of 2 SLE patients and 2 healthy controls.Their ultrastrnctures were examined by transmission electron microscope (TEM).The expression pattern of actin and vinculin was assessed by laser confocal microscopy (LCM).Results MSCs in patients with SLE presented with signs of ageing and lots of autophagosome could be found in most of the cells.F-actin was aggregated and condensed at the:border of cytoplasm.Vinculin was arranged disorderly and condensed in the cytoplasm.Conclusion The change of uhrastructure and cytoskeleton patterns of bone marrow derived mesenchymal cells of SLE patients may play an important role in the abnormal proliferation of these cells in vitro.
