The complete reversal of mulitidrug resistance in leukemia cells by shRNA
- VernacularTitle:shRNA对人白血病K562/ADM细胞多药耐药性的逆转观察
- Author:
Xueqin ZHANG
;
Weidong CAI
- Publication Type:Journal Article
- Keywords:
Multidrug resistance-associated proteins;
Gene,MDR;
RNA interference;
Leukemia
- From:
Chinese Journal of Primary Medicine and Pharmacy
2008;15(5):788-790,插3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the efficiency of reversing MDR by suppressing MDR1 gene with RNAi in K562/ADM cells. Methods For reversing MDR by RNAi technology, two different short hairpin RNAs (shRNAs) were designed and constructed into pGenSil-1 plasmid, respectively. They were then transfected into the highly adriamycin-resistant K562/ADM cell line. The RNAi effect on MDR was evaluated by real-time PCR, and Rhodamine123 (Rh123) efflux assy. Results The stable transfected clones showed varied degrees of reversal of MDR phenotype. Surprisingly, the MDR phenotype was completely reversed in two transfected clones. Conclusion This study demonstrates that MDR can be reversed by the shRNA-mediated RNAi in K562/ADM cells, which provides a valuable clue as to sensitizing multidrug-resistant hepatoma cells to anti-cancer drugs.
