Establishment of HCV infection model in vitro using a human liver cell line 7721
- VernacularTitle:人肝癌细胞系7721丙型肝炎病毒体外感染模型的建立
- Author:
Zhiqiang SONG
;
Fei HAO
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus 7721 cell line Cell culture Cell model Infection
- From:
Chinese Journal of Infectious Diseases
1999;17(4):224-227,285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a cell model that is close to the HCV replication in vivo and can support long-term HCV replication in vitro.Methods A human liver cell line 7721 was inoculated with serum from a chronic hepatitis C patient for 8 hours.After incubation,the presence of HCV RNA,the expression of HCV antigen and the location of HCV RNA in cells and/or supernatant were detected by RTPCR,immunohistochemistry and in situ hybridization respectively.Results It was found that plus-and minus-strand of HCV RNA could be discontinuously detected in both cells and supernatant as late as 66 days after inoculation even if cells had been subcultured for 6 times.HCV NS3、NS5 antigens could be expressed in cells and HCV RNA was mainly located within cytoplasm.Conclusion The above results suggested that 7721 cell line was not only susceptible to HCV but also could support its long-term replication in vitro.This HCV infection model in vitro was proved to be a useful tool for studying the mechanism of HCV infection and replication,as well as evaluating the antiviral compounds and screening the protective antibodies/vaccines.
