The expression of plasmid DNaseI gene in mice bone marrow mesenchymal stem cells
10.3760/cma.j.issn.1007-7480.2009.04.002
- VernacularTitle:小鼠DNA酶真核表达质粒在小鼠骨髓间充质干细胞中的表达
- Author:
Guozhen TAN
;
Qing GUO
;
Ruofei YIN
;
Xiangbin MI
;
Fanqin ZENG
- Publication Type:Journal Article
- Keywords:
Deoxyribonucleases;
Mesenchymal stem cell;
Plasmids;
Mice
- From:
Chinese Journal of Rheumatology
2009;13(4):220-222,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and secretion of mice DNaseI gene plasmid transfected into bone marrow (BM-MSCs) mesenchymal stem cells. Methods The plasmids of mouse DNaseI gene had been transfected into the BM-MSCs of mice by liposomes. The expression of DNaseI gene in the BM-MSCs was detected by western blotting and the DNaseI activity was measured by DNA-methyl green substrate colorimetry. Results About 30% BM -MSCs were transfected with mice plasmid DNaseI gene, DNaseI was expressed in the transfected BM-MSCs and active DNaseI could be detected in the supernatant of cell culture. Conclusion The mice DNaseI gene plasmid can be transfected into mice BM -MSCs by liposomes and DNaseI gene can be expressed by the transfected BM-MSCs and active DNaseI can be secreted. This may provide potential target for the treatment of SLE.
