Preliminary detection of expression changes of human mitochondrial COXI,ND1 and ND6 gene induced by 60Co γ-rays
10.3760/cma.j.issn.0254-5098.2009.02.001
- VernacularTitle:60Co γ射线离体照射诱导人线粒体COXI、ND1和ND6基因表达变化的研究
- Author:
Yuwen LI
;
Jiangbin FENG
;
Xue LU
;
Deqing CHEN
;
Qingjie LIU
- Publication Type:Journal Article
- Keywords:
60Co γ-mys;
Human lymphoblastoid cell line;
Mitochondrion;
Gene expression
- From:
Chinese Journal of Radiological Medicine and Protection
2009;29(2):125-128,142
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of human mitochondrial COXI,ND1 and ND6 genes expression induced by ionizing irradiation.Methods Changes of human COXI,ND1 and ND6 gene expression were detected by RT-PCR and Real-time PCR 8 h after the irradiation in human lymphoblastoid cell lines,which were exposed to 1-10 Gy 60Co γ-rays.And the dose-effect relationships between expression changes of the genes and the doses were analyzed.The changes of these three genes expression were also analyzed at different post-radiation time-points between 0.5 h and 72 h after irradiation of 5 Gy in order to explore the time-effect.Results The expression of three genes COXI,ND1 and ND6,showed either the dose-effect or the time-effect after irradiation.The gene expression levels of three genes up-regulated generally and the peak change time-point was 4 h after irradiation.Conclusion Ionizing radiation,msht induce the changes of mitochondrial gene expression,and the gene expression level is up-regulated.
