Effects of TSPG on apoptosis of K562 cells and the expression of Survivin

VernacularTitle:人参总皂苷对白血病细胞K562凋亡及生存素表达的影响
Author: Donghong WU ; Zhigang YANG ; Qinghua LI
Publication Type:Journal Article
Keywords: Total saponins of panax ginseng; K562 cells; Apoptosis; Survivin
From: Chinese Journal of Primary Medicine and Pharmacy 2009;16(7):1218-1219,1346
CountryChina
Language:Chinese
Abstract: Objective To study the effects of TSPG on apoptosis of K562 cells and the probable mechanism involved. Methods MTT was used to investigate the proliferation of K562 cells;Flow cytometry(FCM) was used to investigate the effects of TSPG on apoptosis of K562 cells; The expression of Survivin in K562 cells treated with differ-ent concentraction of TSPG were examined by RT-PCR. Results The growth of K562 cells was inhibited by TSPG in the concentration dependent manner (P < 0.05). FCM showed that the apoptosis rates of cells in 100 μg/L (23.78%) and 200μg/L TSPG group(33.98%) were higher than those in 101μg/L TSPG group(16.67%), with significant difference(P <0.01 or P < 0.05). The expression rates of Survivin were decreased by the treatment with the increasing concentrations of TSPG (P < 0.05). Conclusion TSPG can restrain the human leukemic cell growth and induce cell apoptosis,which may be related to the decreased expressions of Survivin.