Effect of the serum containing Jiang Zhi Zhuang Gu solution on BMP-2 expression of rat bone marrow stromal cells
10.3760/cma.j.issn.1008-6706.2009.10.001
- VernacularTitle:降脂壮骨方剂含药血清对大鼠骨髓基质细胞骨形态发生蛋白-2表达的影响
- Author:
Quansheng GE
;
Shouyu WANG
;
Yinghui ZHU
;
Weiguo ZHANG
- Publication Type:Journal Article
- Keywords:
Bone morphogenetic proteins;
Cholesterol;
Serum;
Drugs,Chinese herbal;
Bone marrow cells;
Rats
- From:
Chinese Journal of Primary Medicine and Pharmacy
2009;16(10):1729-1730,后插一
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study osteoporosis associated with hyperlipidemia;To research high cholesterol se-rum and Jiangzhizhuanggu-containing serum intervention rat bone marrow stromal cells BMP-2 expression changes. Methods Adult rat femur by density gradient centrifugation separation of bone marrow stromal cells, cultured cells to the third generation, and was identified by flow cytometry. The third generation cells, were randomly divided into three groups. Normal serum control group: two percent blank senun (volume ratio); High cholesterol serum injury group: fi-nal concentration of 4 mmol/L, high cholesterol serum; medicine + pretreatment serum high cholesterol serum injury, group :2% senun Chinese medicine(volume ratio) after pretreatment 2 hours + final concentration of 4 mmol/L, high cholesterol serum. Total RNA was isolated from cells recovered. BMP-2 mRNA expression was detected. And analyzed statistically. Results (1) Blank serum compared with the control group,high cholesterol serum cultured bone mar-row stromal cells proliferation was inhibited. BMP-2 mRNA expression significantly decreased(P < 0.01) . (2) Jiang-zhizhuanggu solution containing serum protection two hours later to join a high cholesterol serum group, the cell growth conditions improved significantly, BMP-2 mRNA expression significantly increased than pure high cholesterol serum injured group(P <0.01) . Conclusion Solution's Jiangzhizhuanggu containing serum can enhance high cholesterol serum environment bone manow stromal cells BMP-2 mRNA expression, significantly improved bone marrow stromal cell growth state is conducive to the treatment of osteoporosis.
