Establishing of the HSV-2 infected cell culture system
10.3760/cma.j.issn.1008-6706.2010.01.005
- VernacularTitle:单纯疱疹病毒2型感染的细胞培养系统的建立
- Author:
Yi LIANG
;
Youkun LIN
;
Ming LIANG
- Publication Type:Journal Article
- Keywords:
Herpes virus 2;
Cell culture techniques
- From:
Chinese Journal of Primary Medicine and Pharmacy
2010;17(1):11-13,插6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HSV-2 infected cell culture system,so as to confirm the suppressing effect of medicine on HSV-2 including effective concentration and safety.Methods A monkey kidney cell line,Vero ,and a human tracheal epithelial cell line,Hep-2 were used in HSV infected cell culture system.HSV-2 was propagated in Vero cells and Hep-2 cells was served as targets of HSV-2 infection.Then cytopathic effect(CPE) was performed.Results HSV-2 was propagated abundance in Vero cells after4 days:The CPE occurred in Hep-2 cells after 12 hours infected by HSV-2.Conclusion The HSV-2 infected cell culture system we established was stable for utilization.As well as to confirm the suppressing effect of medicine on HSV-2.
