Protection of human osteoprotegerin mediated by adeno-associated virus on joint destruction in collagen-induced arthritis
10.3760/cma.j.issn.1007-7480.2010.02.010
- VernacularTitle:腺相关病毒介导的人护骨素对炎症性关节破坏的保护作用
- Author:
Lizhi BAO
;
Xinghai HAN
;
Dongbao ZHAO
;
Jianlong GUAN
;
Shengming DAI
;
Qing CAI
;
Yeqing SHI
;
Lanlin ZHANG
;
Jing LIU
- Publication Type:Journal Article
- Keywords:
Arthritis,experimental;
Arthritis,rheumatoid;
Cytokines
- From:
Chinese Journal of Rheumatology
2010;14(2):98-101,后插一
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using an in vivo adeno-associated virus(AAV)-mediated gene transfer technique,this study was designed to evaluate the protective effects of human osteoprotegerin(OPG)transgene against joint destruction in collagen induced arthritis(CIA)model.MethodsAfter CIA was established in the Sprague-Dawley rats,the experimental animals were treated with PBS or rAAV-EGFP or rAAV-hOPG (100μl/d)intra-articular injection 25 days after arthritis induction for 10 days.Paraffin-embedded joints were then analyzed histologically.The joint destruction was evaluated by Larsen Score.The protein expression of OPG,IL-1,MMP-3 was identified by enzyme-linked immunosorbent assay(ELISA).Results Suecessful trans-gene expression was confirmed by the detection of OPG by ELISA and positive fluorescence of the frozen joint section. Image analysis revealed that the expression of OPG significantly protected against joint destruction by 30% compared with the CIA group. Conclusion OPG gene transfer mediated by rAAV effectively protects against bone destruction induced by CIA model. Those data suggest that gene transferring using rAAV-OPG may be a feasible and effective therapeutic approach to treat or prevent joint destruction in inflammatory arthritis.
