Using damaged cardiomyocytes onditional induction culture medium to induce the differentiation of human bone marrow mesenchymal stem cells

VernacularTitle:心肌细胞损伤条件诱导液诱导人骨髓间充质干细胞的分化
Author: Fangge DENG ; Yulin LI ; Yingzhi MA
Publication Type:Journal Article
Keywords: Human mesenchymal stem ceils; Cardiomyo-like cells; Cell differentiation; Conditionally induced culture medium
From: International Journal of Biomedical Engineering 2009;32(6):325-327,封3
CountryChina
Language:Chinese
Abstract: Objective To investigate the possibility of conditionally induced culture medium (CICM) of oxidative damaged cardiomyocytes(CMs) derived from SD suckling rats to induce the differentiation of human bone marrow mesenchymal stem cells (hMSCs) into cardiomyo-like cells. Methods hMSCs and SD suckling rats' CMs were respectively isolated and cultured in vitro. Beating CMs were damaged by H_2O_2 and the the resultant culture medium wherein the oxidative damaged CMs were euhivated was used as CICM. Passaged hMSCs were induced by CICM for 4 weeks. The morphologies of induced hMSCs were observed by inverted microscope and the special cardio-markers-Cardiac troponin I(cTnI) and desmin were identified by immunocytochemistry method. Results After coincubation with CICM, hMSCs became larger and expressed cTnI and desmin.Conclusion CICM of oxidative injured CMs could induce hMSCs differentiation into cardiomyo-like cells.