Effect of exogenesis vascular endothelial growth factor gene on the apoptosis of brain cells in the HIBD of newborn rats

VernacularTitle:外源性血管内皮生长因子基因对新生鼠缺氧缺血性脑损伤脑细胞凋亡的影响
Author: Hong JI ; Lixin XU ; Yunxia QU ; Zhongbo WANG
Publication Type:Journal Article
Keywords: Hypoxic-ischemic brain damage,Vascular endothelial growth factor; Newborn rat
From: Chinese Pediatric Emergency Medicine 2010;17(3):-
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of exogenesis VEGF 120 gene on the apoptosis of brain cells in the HIBD of newborn rats. Methods VEGF eukaryotic expression plasmid (pCDNA 3.1/r VEGF 120) was constructed by cloning rat VEGF 120 cDNA into eukaryotic expression vector pCDNA 3.1. The HIBD model was established with seven days old SD rats,and all rats were diveded into two groups at random :contral group 18 rats( every rat model was injected pCDNA 3.1 100 μg immediately after hypoxia-is-chemic.then raised seven days) and therapy group 18 rats (every rat model was injected pCDNA 3.1/ rVEGF 120 100 μg immediately after hypoxia-ischemic). Flow cytometer( FCM) was used to detect the ratio of apoptosis of brain cell. Results There was a significant decrease in the ratio of apoptosis brain cells( control group 17.505 ± 0.949; therapy group 8.93 ± 0. 332). Conclusion The VEGF gene product can reduce apoptosis of brain cells.