Isolation, Purification and Characterization of Human Serum Apolipoprotein A Ⅰ
10.3760/cma.j.issn.1008-6706.2010.14.033
- VernacularTitle:人载脂蛋白AⅠ的分离纯化
- Author:
Zhaoping LIU
;
Lili LIU
;
Jiashan SHEN
;
Xiangkun HE
;
Shen XIANG
;
Zhiwu AI
- Publication Type:Journal Article
- Keywords:
Apolipoprotein A Ⅰ(apoA I);
Isolation;
Purification
- From:
Chinese Journal of Primary Medicine and Pharmacy
2010;17(14):1937-1938,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a method to separate and purify apolipoprotein Ⅰ from human serum conveniently and efficiently. Methods Apolipoprotein Ⅰ was separated and pufified by ultracentrifugation and affinity chromatography. Then the purified apolipoprotein Ⅰ was analyzed by SDS-polyacrylamide gel electrophoresis and agar gel double immunodiffusion test. Results The purified apolipoprotein Ⅰ was satisfactory. Conclusion This method had good reliability and was convenient and economical.
