High levels of adiponectin in the inflammatory joint of rheumatoid arthritis contribute to elevated interleukin-6, monocyte chemoattractant proteins-1 and receptor activator for nuclear factor κB ligand expression
10.3760/cma.j.issn.1007-7480.2010.09.003
- VernacularTitle:类风湿关节炎炎性关节中高水平脂联素促进白细胞介素-6单核细胞趋化因子-1和核因子-κB受体活化因子配体表达
- Author:
Wenfeng TAN
;
Lingxiao XU
;
Fang WANG
;
Dunming GUO
;
Ting LIU
;
Shaoheng HE
;
Miaojia ZHANG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Interleukin-6;
Monocyte chemoattractant proteins
- From:
Chinese Journal of Rheumatology
2010;14(9):592-596,后插一
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential role of high levels of adiponectin (AD) in the inflammatory joint of rheumatoid arthritis (RA). Methods ELISA was used to measure the levels of AD, IL-Iβ,IL-6, IL-8, TNF-α, MCP-1 and MMP-9 in the synovial fluids of RA and osteroarthritis (OA), the levels of these cytokines were tested after the synovial fibroblasts (SFLs) were stimulated with AD. Doublelabeling immunohistochemistry was used to analyze the expression of AD in RA synovium. Cytokines were measured by ELISA after SFLs were stimulated with AD. The expression of RANKL was detected by real-time PCR after MH7A were treated with AD and IL-6 ANOVA, Student's t-test, Mann-Whitney U-tese, Spearman's-test were used for statistical analysis. Results High levels of AD in RA synovial fluids were correlated with IL-6 levels. Double labeling immunohistochemistry showed that AD was localized in fibroblasts. MCP-1 and IL-6 were dramatically increased in human synovial fibroblasts following incubation with recombinant AD for 24 h. RANKL mRNA was significantly increased in MH7A after treated with AD and IL-6. Conclusion High levels of AD in the inflammatory joints of RA are likely to contribute to the high expression of IL-6, MCP-1 and RANKL, which may play an important role in the chronic inflammation, osteoclasts activation and bone erosion in RA.
