Effects of midazolum-ketamine oral solution on the expression of NMDA receptor 1 and GABA A receptor mRNA in cerebral cortex of rat
10.3760/cma.j.issn.1673-4912.2010.04.019
- VernacularTitle:咪达唑仑和氯胺酮口服液对大鼠大脑氮-甲基-D-天冬氨酸受体1和γ-氨基丁酸a受体的影响
- Author:
Jin ZHANG
;
Sheng HAN
;
Ying WANG
- Publication Type:Journal Article
- Keywords:
Midazolum-ketamine oral solution;
Cerebral cortex;
NMDAR1;
GABAAR;
Rat
- From:
Chinese Pediatric Emergency Medicine
2010;17(4):338-340,后插5
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study examined the effects of Midazolum-ketamine oral solution (MKOS) on the expression of N-methyl-D-aspartate receptor 1 (NMDAR1) and gamma-aminobutyric acid (GABA)A receptors (GABAAR) mRNA in the cerebral cortex of rat, in order to investigate the sedation mechanism of MKOS. Methods Fifty Sprague-Dawley(SD) rats were divided into ten groups according to the observed time after MKOS administration (0,5,10, 15,30,60,90,120,240 and 360 minutes, n =5 each). The 0 minute group(control group) received 0.9% saline instead. Immunohistochemical staining and in situ hybridization were used to detect the expressions of NMDAR1 and GABAAR mRNA in the cerebral cortex. Results Both GABAAR and NMDAR1 all expressed in the glial cells of cerebral cortex. The expression of NMDAR1 in control group was strong. The expression of NMDAR1 became weaker during 15 to 90 minutes after administration of MKOS (P＜0.05). The expression of GABAAR mRNA in control group was weaker,while became stronger during 30 to 90 minutes after administration of MKOS (P ＜0. 05). Conclusion MKOS may play sedation by strengthening the expression of GABAAR and suppressing the expression of NMDAR1 in the cerebral cortex.
